Histone H2AvD pS137 antibody orb345531 from Biorbyt

Product Specs
ItemHistone H2AvD pS137 antibody
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb345531
Quantity100 µg
ApplicationsELISA, IHC, WB
Conjugate/TagUnconjugated
PurityAffinity purified Anti-Histone H2AvD pS137 Antibody is directed against the phosphorylated form of Drosophila H2AvD protein at the pS137 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against Drosophila H2AvD pS137 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA. A BLAST analysis was used to suggest little to no cross reactivity with H2AvD proteins from other sources based on a comparison using the immunizing sequence. Reactivity against homologues from other sources is not known
TargetHistone H2AvD pS137
IsotypeIgG
ImmunogenHistone H2AvD pS137 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 125-141 of Drosophila melanogaster (fruit fly) H2AvD protein
NCBI Full Gene NameHistone H2A variant
NCBI Gene AliasesH2avD, gammaH2AV, H2A.F/Z, dHis2Av, Hist, 5499, HIS2AVD, H2av, gammaH2Av, His, H2A.X, H2A.Z, His2av, H2A.V, gamma-HIS2AV, *i H2av, gammaH2Ax, His2A, HIS2AV, His2AvD, h2AvD, His2AV, H2AV_DROM, gamma-H2AvD, his, gamma-H2AV, l(3)05146, H2a.V, gamma-His2Av, H2Av, Hist2av, HIS, P-H2Av, His2, l(3)97Dd, l(3)810, gammaH2ax, l(3)L1602, H2AvD, H2AX, H2AZ, gamma-H2Av, HisH2Av, Dmel\CG5499, H2AV, his2Av, CG5499, H2A, his2av
ClonalityPolyclonal
HostRabbit
Concentration1.1 mg/ml
ReactivityDrosophila
FormatLiquid (sterile filtered)
StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use

Specifications/Features

Application Notes: Histone H2AvD pS137 Antibody is tested in ELISA, Immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 14 kDa in size corresponding to phosphorylated H2AvD protein by western blotting in the appropriate Drosophila tissue or cell lysate or extract. Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS137 of H2AvD protein.

Application Dilutions: ELISA: 1:29,000 - 1:30,000, IHC: 2 ug/ml, WB: 1:400 - 1:1,600

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